Mini-Prep Isolation of Plasmid DNA

£115.00

670

Small-scale rapid isolation of plasmid DNA is a routine procedure used for screening and analysis of recombinant DNAs in cloning and subcloning experiments. In this experiment, students isolate plasmid DNA without the use of toxic chemicals such as phenol or chloroform.

• Explore plasmid DNA and key biotechnologies
• Illustrate how electrophoresis separates molecules by shape and size
• Extract and purify plasmid DNA from our classroom-friendly strain of E. coli cells
• Run gel electrophoresis on extractions and visualize different plasmid configurations
• Plasmid DNA is highly stable, easy-to-see, and creates multiple bands on a gel

Kit Includes:

Instructions, Plasmid LyphoCells™, Tris Buffer Concentrate, Sodium Hydroxide Solution, SDS Solution, Resuspension Buffer, Potassium Acetate Solution, RNase Solution, UltraSpec-Agarose™, 10X Gel Loading Solution, 50X Concentrated Electrophoresis Buffer, Practice Gel Loading Solution, FlashBlue™ DNA Stain, InstaStain™ Blue Cards, Disposable Pipets, & Microcentrifuge Tubes.

All You Need:

DNA Electrophoresis, Micropipettes: 5-50 µl, Microcentrifuges, White Light Box, Microwave or Hot Plate, Isopropanol, & 70% Ethanol.

*PLEASE NOTE WE ONLY SUPPLY CHEMICALS TO EDUCATIONAL ESTABLISHMENTS *

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Mini-Prep Isolation of Plasmid DNA

£115.00

£115.00

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